Vesicle detection

Extracellular vesicles (EVs) are small particles surrounded by a lipid bilayer. Most cells are capable of secreting EVs. They carry cargos between cells and play roles in diverse processing from embryo development to injury response, and from angiogenesis to disease progression. The typical size of EVs is in the range from 50-1000 nm, and TEM is the most convenient instrument for their visualization. When analyzed by conventional TEM with heavy metal staining, most EVs exhibit a characteristic cup-shaped morphology. Here you can automatically detect and measure EVs in your images

Detection is based on the Mask R-CNN. Each particle is identified, labeled, and measured. The green lines show the detected edges of the particles, and the red lines show the ellipses used for approximation.

The measurement results are stored in a .csv file with the following parameters:

Name – the file name
Id – the particle number
Score – the score of the particle, which is an estimate of the probability that this particle is a vesicle
Area – particle area, measured in pixels
Perimeter – particle perimeter, measured in pixels
Roundness – a value in the range from 0 to 1, a measure of the particle roundness, calculated as Roundness = 4π(S/p2)
Ellipse_area, a, b, and a+b are four parameters related to the ellipse approximating the detected particle. The values a and b are the semi-axes of the ellipse, and a+b is a relevant estimate of the particle size. All the parameters measured in pixels
On_edge – a Boolean parameter indicating whether the particle touches the edge of the image

Dataset and code available here

Legal Statement
Last updated: November 7, 2020
While using this tool, we don’t ask you to provide us personally identifiable information, as well as any information about your images. We do not use Cookies or similar tracking technologies to track the activity on our tool.
After you upload your images, you will get a unique link, which can be used to access the results of the processing. This link will be available for at least a month; we cannot guarantee the storage of your images for a longer period. We do not use the uploaded images for any purposes.
This tool is provided free of charge. If you use it, please cite as Igor Nikishin, Ruslan Dulimov, Gleb Skryabin, Sergey Galetsky, Elena Tchevkina, Dmitry Bagrov “The neural network-based online tool for the automated detection of extracellular vesicles in TEM images” // Micron, 2020
We make the greatest efforts for the maintenance of this online tool, however, can not guarantee the absence of technical failures. We do not take any responsibility for any incorrect use of our tool, as well as any results obtained with the help of this tool.
Contact information
We appreciate any comments and questions about Bioeng.ru/scanev and its functions. You can contact us by email: bagrov@mail.bio.msu.ru
License
We provide our software via the MIT License, which is a permissive license with minimum restrictions. The core of our tool is Mask R-CNN, which is also distributed via the MIT License. The License is available here.